The invention is to improve the storage stability of metarhizium anisopliae conidia. First to construct the disturb carrier of neuter trehalase RNA of metarhizium anisopliae conidia, then to found the transform system of conidiophore. Appraising by DNA imprinted, detecting the expression of its neuter trehalase gene by the RNA imprinted. Compare to the inner trehalose content, storability, heat tolerance between the transformant and the departure bacterial strain sporeú¼it can get that there is prodigious difference in the inner trehalose content, storability, heat tolerance between them. The storability, heat tolerance of the Metarhizium anisopliae conidia can be improved by this method.