This invention relates to oligonucleotide prime, mandarin orange yellow dragon germ real time fluorescence PCR detecting probe, solid phase reagent and its detecting method of mandarin orange yellow dragon germ detecting. It is a special quick detecting method direct towards mandarin orange yellow dragon disease, there is only 3 hours from sampling to detection, and detecting sensitivity is high, specificity is strong, stable and reliable. Misjudging in existing normal diagnosis method, missed diagnosis in electrical glass by asymmetry of germ and hard antibody preparation in single clone antibody and lack of specific reaction are conquered by this method, and false negative and false positive of normal PCR are avoided. Operation of solid phase kit is convenient, and its using is simple, and detecting sensitivity is high, specificity is strong, stable and reliable. So it is propitious to port detection of mandarin orange of import and export. Also it is propitious to allocation and transportation of mandarin orange inoculation, scion and other asymptomatic germ material, and early stage warning detection of non epidemic-stricken area orange yellow dragon disease.