The invention relates to a wiring structure of printed circuit board, wherein said printed circuit board comprises several high-speed differential signal transmission lines on one signal layer and several guide holes connected to the lines; the lines are two transmission lines with constant distance, same length, and reverse signal directions; the guide holes are asymmetry arranged at two sides of transmission lines, while the horizontal distance between each couple guide holes is A; T is the ascending time of signal; V is signal transmission speed; the inventive wiring structure can make the resistance reach best match, reduce signal reflection, and improve the integration of differential signal.