This invention relates to a method for preparing edible fungi nutrient liquid with wood vinegar as the major component. The nutrient liquid is composed of: wood vinegar 12-20 parts, amino acids 4-8 parts, urea 5-10 parts, monopotassium phosphate 5-10 parts, sugar 4-8 parts, monosodium glutamate 0.2-1 part, vitamin B1 0.2-1 part, boric acid 3-6 parts, zinc sulfate 3-6 parts, magnesium sulfate 5-9 parts, and water 40-50 parts. The method comprises mixing the above components in a container, stirring and filtering. The nutrient liquid is rich in nutrients needed for edible fungi, thus can promote the growth of hypha, increase the productivity (by 20-30%), improve the resistance to disease, and improve the quality.