The utility model discloses a rotary diffused fluorescence tomngraphic system which includes a semi-conductor laser, a computer, a rotary working platform, a stepper motor and a CCD camera. The semi-conductor laser and a semi-conductor refrigerating box are respectively fixed on two horizontal arms of the rotary working platform; the CCD camera is positioned in the semi-conductor refrigerating box; a sample clamp is fixed on a support and positioned between the CCD camera and the semi-conductor laser; the stepper motor drives the CCD camera and the semi-conductor laser to rotate around the central point of a vertical rod; the computer transmits control signal to the rotary working platform and the CCD camera, receives the image data transmitted from the CCD camera, analyzes and processes the image data. The system of the utility model can control the quantity of the collected data randomly and improve the imaging effect; can realize rotation of the stepper motor and control of photography of the refrigeration charge coupled camera at the same time. In this way, the refrigeration charge coupled camera can stably and effectively collect data during rotation of the system.