The utility model discloses a sensitive amplifier for EEPROM and a reading circuit structured by the amplifier. The sensitive amplifier comprises a charging control circuit, a detection circuit and a holding shaping output circuit; the charging control circuit is composed of two same charging control sub-circuits; the detection circuit is an XNOR (exclusive NOR) gate; the holding shaping output circuit holds and shapes the output of the detection circuit to forma a standard digital electric level, the reading circuit being composed of the sensitive amplifier comprises a first storage module and a second storage module that are completely symmetric, and two bit lines of each sensitive amplifier are respectively connected onto the corresponding bit lines of the first and second storage modules. The sensitive amplifier has simple circuit structure without biasing circuit, the occupied area is small, the reading speed is fast, the dynamic power consumption is low, the static power consumption is almost zero, and the operation voltage range is large; the reading circuit being composed of the sensitive amplifier has the advantages of stable performance and resisting the degradation of component property.