The utility model relates to a choke valve for a puncture device, which has high sealing property and has low resistance when being penetrated by surgical instruments. The choke valve is in sealing fit on a working channel in the puncture device and comprises a cup body part made of elastic materials, wherein the bottom surface of the cup body part is a plane; a crossed gap is formed in the center of the bottom surface; four arc-shaped grooves opposite to four included angles of the crossed gap are formed in the side wall of the cup body part; column-shaped surgical instruments are suitable for penetration of the crossed gap; the bottom surface of the cup body part is suitable for sealing the working channel when not being penetrated by the surgical instruments. The bottom surface of the cup body part is in sliding fit with the column-shaped surgical instrument through the crossed gap; relative to a crack structure of a duck-billed choke valve, material deformation is slight and corresponding resistance applied to the surgical instruments is reduced.