The utility model discloses a tire fixing device used for a tire gluing machine. The tire fixing device used for the tire gluing machine comprises a box body, a driving air cylinder, at least two connection rods, at least two supporting rods and a control panel, wherein the driving air cylinder is horizontally arranged in the middle of the box body; the end parts of extending rods of the driving air cylinder are respectively hinged with one end of each connection rod; the other ends of the connection rods are hinged with one end of each supporting rod; annular bumps are arranged at the other ends of the supporting rods; the supporting rods pass through the lateral face of the box body and extend out from the outside of the lateral face of the box body; the control panel is arranged at the upper part of the front face of the box body, and controls the driving air cylinder to act. The connection rods are driven to act by controlling the driving air cylinder through the operation on the control panel; the connection rods drive the supporting rods to move upward and downward, so that the annular bumps at the end parts of the supporting rods are clamped with or release the edge of an inner ring of a tire. Therefore, the tire is fixed or released. Meanwhile, the tire can be rapidly fixed. After a gluing process, the tire fixing device is convenient to dismount; the tire is firmly fixed, so that the purposes of reasonable design, simple structure and good application effect can be realized.