The utility model discloses a glass substrate cleaning machine transmission shaft bearing frame, including the base with form at this base upper bearing installation department, wherein, be formed with the retaining cavity in the bearing installation department, the transmission shaft bearing sets up in this retaining cavity, is provided with water inlet and delivery port with the cavity intercommunication that impounds on the surface of bearing installation department. Water in the retaining cavity can carry out lubricated wearing and tearing in order to lighten the bearing effectively to the bearing, and the dust particle that the bearing wear produced can be taken out of bearing frame to the water that flows simultaneously to avoided dust particle to produce secondary pollution attached to glass substrate to the product.