Time -resolved fluorescence immunoassay kit based on FPSA magnetic particle, it includes the box body, being used for of being connected with the box body is right the box body carries out the confined lid, and the box body bottom is equipped with the fixed plate for vertical placing strengthens liquid reagent bottle, concentrated washing liquid reagent bottle and reacts the first recess of buffer reagent bottle respectively, and is used for vertical peridium FPSA monoclonal antibody's magnetic particle reagent bottle, FPSA's calibration article reagent bottle and the second recess of europium mark FPSA monoclonal antibody solution reagent bottle placed respectively, the internal surface of lid is equipped with the spout, and the guide rail that sets up on spout and the micropore board corresponds, and the guide rail slides in the spout internal fixation micropore board, shown kit provide a reaction system who is close the homogeneous phase, compare with prior art, the utility model discloses the kit have facilitate the use, easy operation, stability be good, and higher detectivity and specificity, detects more accurate reliably to the performance parameter of preferred has been reached.