The utility model discloses a vacuum filtration application of sample equipment. This vacuum filtration application of sample equipment includes: the application of sample unit, the application of sample unit includes: the sample application device, be provided with at least one sample application hole on the sample application device, suction filter device, suction filter device includes filter membrane and filter membrane supporting layer, the filter membrane supporting layer sets up the filter membrane below, the filter membrane only supports the below of sample application device, be provided with on the filter membrane supporting layer with the suction filtration hole that sample application hole one -to -one set up, and surplus sample storage device, surplus sample storage device with suction filter device links to each other, and vacuum unit, vacuum unit with surplus sample storage device links to each other. From this, can utilize sample application hole and suction filtration hole as the template of sample flow through the filter membrane, utilize vacuum unit to provide the power that sample flow moved to can form the edge on the filter membrane does not have diffusion, spot color and luster homogeneous and the high sample spot of repeatability, and can improve the speed of system appearance.