The utility model discloses a people coronavirus NL63 detect reagent box, include: the box body, the box body is cuboid, square or cylinder, with the box body rotates the lid of connecting, the setting is in soft cushion board in the box body, the soft cushion board is kept away from one side of box body bottom is equipped with first recess, second recess, third recess and fourth recess, and with the first reagent pipe that first groove phase matches, with the second reagent pipe that the second groove phase matches, with the third reagent pipe that the third groove phase matches, with the fourth reagent pipe that the fourth groove phase matches, first reagent is effective presorts dress PCR reaction liquid in the take up, is second reagent effective in bearing RT the PCR enzyme, third reagent is effective in bearing the positive article of accusing, fourth reagent is effective in bearing the negative article of accusing. This kind of people coronavirus NL63 detect reagent box easy operation, practicality are strong, and whether the sample median that can the short -term test play awaits measuring contains people coronavirus NL63.