The utility model provides a constant voltage cell pressurization culture apparatus for cultivate the cell among the cell culture dish under stable static force, its characterized in that includes: cell culture box for place cell culture dish, the top has the opening that supplies cell culture dish to pass through, is provided with air filler valve and drain tap on the box, and the barometer is used for the real -time measurement and shows the interior atmospheric pressure of cell culture box, and sealed constant voltage unit, install the atmospheric pressure in opening and regulatory cell incubator are sealed to the opening part, wherein, sealed constant voltage unit has piston assembly and a vertical pressurizing assembly who installs in open -top department, piston assembly have with opening phase -match gliding pressurizing piston from top to bottom, vertical pressurizing assembly sets up and is adding the top land for a gas, vertical pressurizing assembly includes a plurality of weight and transfers water saving box that in the vertical downward pressure of the compression cell culture box is provided.