发明公开
- 专利标题: Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor
- 专利标题(中): 酶免疫法和肽酶联合法,其柠檬酸盐,抗体和其他药物
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申请号: EP83111727申请日: 1981-03-28
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公开(公告)号: EP0111216A3公开(公告)日: 1985-01-16
- 发明人: Iwasa, Susumu , Yoshida, Isamu , Kondo, Koichi
- 申请人: Takeda Chemical Industries, Ltd.
- 专利权人: Takeda Chemical Industries, Ltd.
- 当前专利权人: Takeda Chemical Industries, Ltd.
- 优先权: JP4248480 19800331; JP8046780 19800613; JP450781 19810114
- 主分类号: C07G07/00
- IPC分类号: C07G07/00 ; G01N33/54 ; G01N33/58 ; G01N33/76 ; C12N09/96 ; C07C103/52
摘要:
In an enzyme immunoassay when a specific antibody produced by contacting a peptide essential to the formation of a specific antibody to a peptide antigen, a freeze-dried material of β-D-galactosidase-enzyme conjugate or a peptide-enzyme conjugate prepared by coupling a labeling enzyme with a peptide of the general formula: wherein R, is a peptide fragment consisting of 1 to 14 amino acid residues including Gly in the 14-position of the peptide Ala 1 -Pro 2 -Pro 3 -Pro 4 -Ser 5 -Leu 6 -Pro 7 -Ser 8 -Pro 9 -Ser 10 -Arg 11 -Leu 12 -Pro 13 -Gly 14 is used, a high reproducibility of the result of the enzyme immunoassay is obtained.
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