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    Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor
    1.
    发明公开
    Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor 失效
    Enzymimmunologisches Testverfahren，Peptid-Enzym-Konjugat，dessen Lyophilisat，Antikörperund Reagenzsatzdafür。

    公开(公告)号：EP0111216A2

    公开(公告)日：1984-06-20

    申请号：EP83111727.0

    申请日：1981-03-28

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Iwasa, Susumu ,  Yoshida, Isamu ,  Kondo, Koichi

    IPC分类号： C07G7/00 ,  G01N33/54 ,  G01N33/58 ,  G01N33/76 ,  C12N9/96

    CPC分类号： C12N9/96 ,  C07K14/59 ,  C07K16/26 ,  G01N33/531 ,  G01N33/535 ,  G01N33/76

    摘要： In an enzyme immunoassay when a specific antibody produced by contacting a peptide essential to the formation of a specific antibody to a peptide antigen, a freeze-dried material of β-D-galactosidase-enzyme conjugate or a peptide-enzyme conjugate prepared by coupling a labeling enzyme with a peptide of the general formula: wherein R, is a peptide fragment consisting of 1 to 14 amino acid residues including Gly in the 14-position of the peptide Ala 1 -Pro 2 -Pro 3 -Pro 4 -Ser 5 -Leu 6 -Pro 7 -Ser 8 -Pro 9 -Ser 10 -Arg 11 -Leu 12 -Pro 13 -Gly 14 is used, a high reproducibility of the result of the enzyme immunoassay is obtained.

    摘要翻译： 在通过使形成特异性抗体所必需的肽与肽抗原接触而产生的特异性抗体的酶免疫测定中，β-D-半乳糖苷酶 - 酶偶联物的冷冻干燥物质或通过将 用具有以下通式的肽标记酶：H-R1-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-OH其中R1是由1至14个氨基酸残基组成的肽片段，包括Gly 肽Ala 1 - Pro 2 - Pro 3 - Pro 4 - Ser 5 - Leu 6 - 位7-Ser 8 -Pro 9的14位 > -Ser 1 <0> -Arg <1> -Leu <1> 2 -Pro 1 <3> -Gly <1> 4，结果的重现性高 的酶免疫测定。

    Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor
    4.
    发明公开
    Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor 失效
    酶免疫法和肽酶联合法，其柠檬酸盐，抗体和其他药物

    公开(公告)号：EP0111216A3

    公开(公告)日：1985-01-16

    申请号：EP83111727

    申请日：1981-03-28

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Iwasa, Susumu ,  Yoshida, Isamu ,  Kondo, Koichi

    IPC分类号： C07G07/00 ,  G01N33/54 ,  G01N33/58 ,  G01N33/76 ,  C12N09/96 ,  C07C103/52

    CPC分类号： C12N9/96 ,  C07K14/59 ,  C07K16/26 ,  G01N33/531 ,  G01N33/535 ,  G01N33/76

    摘要： In an enzyme immunoassay when a specific antibody produced by contacting a peptide essential to the formation of a specific antibody to a peptide antigen, a freeze-dried material of β-D-galactosidase-enzyme conjugate or a peptide-enzyme conjugate prepared by coupling a labeling enzyme with a peptide of the general formula: wherein R, is a peptide fragment consisting of 1 to 14 amino acid residues including Gly in the 14-position of the peptide Ala 1 -Pro 2 -Pro 3 -Pro 4 -Ser 5 -Leu 6 -Pro 7 -Ser 8 -Pro 9 -Ser 10 -Arg 11 -Leu 12 -Pro 13 -Gly 14 is used, a high reproducibility of the result of the enzyme immunoassay is obtained.

    摘要翻译： 在通过使形成特异性抗体所必需的肽与肽抗原接触而产生的特异性抗体的酶免疫测定中，β-D-半乳糖苷酶 - 酶偶联物的冷冻干燥物质或通过将 用具有以下通式的肽标记酶：H-R1-Pro-Ser-Asp-Thr-Pro-Ile-Leu-Pro-Gln-OH其中R1是由1至14个氨基酸残基组成的肽片段，包括Gly 肽Ala 1 - Pro 2 - Pro 3 - Pro 4 - Ser 5 - Leu 6 - 位7-Ser 8 -Pro 9的14位 > -Ser 1 <0> -Arg <1> -Leu <1> 2 -Pro 1 <3> -Gly <1> 4，结果的重现性高 的酶免疫测定。