发明公开
- 专利标题： A process for the production of purified glucose isomerase
- 专利标题（中）： 一种用于纯化的葡萄糖异构酶的制备方法。
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申请号： EP85116277.6申请日： 1985-12-19
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公开(公告)号： EP0185379A2公开(公告)日： 1986-06-25
- 发明人: Johnson, Richard A. , Antrim, Richard L. , Lloyd, Norman E.
- 申请人： NABISCO BRANDS, Inc.
- 申请人地址： Nabisco Brands Plaza Parsippany New Jersey 07054 US
- 专利权人： NABISCO BRANDS, Inc.
- 当前专利权人： NABISCO BRANDS, Inc.
- 当前专利权人地址： Nabisco Brands Plaza Parsippany New Jersey 07054 US
- 代理机构： Eitle, Werner, Dipl.-Ing.
- 优先权： US684204 19841220
- 主分类号： C12N9/92
- IPC分类号： C12N9/92
摘要：
The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.
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