发明公开
EP0278706A2 Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
失效
的重组质粒用于L-苯丙氨酸解氨酶和含有它转化株的表达。
- 专利标题： Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
- 专利标题（中）： 的重组质粒用于L-苯丙氨酸解氨酶和含有它转化株的表达。
-
申请号： EP88301011.8申请日： 1988-02-08
-
公开(公告)号： EP0278706A2公开(公告)日： 1988-08-17
- 发明人: Fukuhara, Nobuhiro , Yoshino, Setsuo , Sone, Satori , Nakajima, Yoshiyuki , Makiguchi, Nobuyoshi
- 申请人： MITSUI TOATSU CHEMICALS, Inc.
- 申请人地址： 2-5 Kasumigaseki 3-chome Chiyoda-Ku Tokyo 100 JP
- 专利权人： MITSUI TOATSU CHEMICALS, Inc.
- 当前专利权人： MITSUI TOATSU CHEMICALS, Inc.
- 当前专利权人地址： 2-5 Kasumigaseki 3-chome Chiyoda-Ku Tokyo 100 JP
- 代理机构： Holdcroft, James Gerald, Dr.
- 优先权： JP24705/87 19870206; JP152357/87 19870618
- 主分类号： C12N15/60
- IPC分类号： C12N15/60
摘要：
A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P L promoter of the lambda phage, the tac promoter and the P L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.
公开/授权文献
信息查询
IPC分类:
