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    Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
    1.
    发明公开
    Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same 失效
    的重组质粒用于L-苯丙氨酸解氨酶和含有它转化株的表达。

    公开(公告)号：EP0278706A2

    公开(公告)日：1988-08-17

    申请号：EP88301011.8

    申请日：1988-02-08

    申请人： MITSUI TOATSU CHEMICALS, Inc.

    发明人： Fukuhara, Nobuhiro ,  Yoshino, Setsuo ,  Sone, Satori ,  Nakajima, Yoshiyuki ,  Makiguchi, Nobuyoshi

    IPC分类号： C12N15/60

    CPC分类号： C12N9/88 ,  C12N15/70

    摘要： A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P L promoter of the lambda phage, the tac promoter and the P L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.

    摘要翻译： 苯丙氨酸氨 - 裂合酶（PAL）中的表达的重组质粒是通过在其中掺入trp启动子零下35区域和lac启动UV-5启动子减去构成的组合启动子包含：（a）融合启动子（tac启动子）构建 10区域和（b）的λ噬菌体的PL启动子，tac启动子和PL启动子被连接以便具有相同的方向性。 PAL的在大肠杆菌中此重组质粒允许更有效的表达。

    A method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby
    4.
    发明公开
    A method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby 失效
    一种用于通过在用于生产外源基因产物的培养基和方法的糖浓度的控制来调节外源基因表达的方法。

    公开(公告)号：EP0279664A2

    公开(公告)日：1988-08-24

    申请号：EP88301355.9

    申请日：1988-02-18

    申请人： MITSUI TOATSU CHEMICALS, Inc.

    发明人： Fukuhara, Nobuhiro ,  Yoshino, Setsuo ,  Sone, Satori ,  Nakajima, Yoshiyuki ,  Makiguchi, Nobuyoshi

    IPC分类号： C12N15/71 ,  C12N9/88 ,  C12N15/72 ,  C12N15/73

    CPC分类号： C12N15/70 ,  C12N9/88

    摘要： Escherichia coli carrying a hybrid plasmid constructed by inserting a desired foreign gene into an expression vector, was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process to suppress the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.

    摘要翻译： 大肠杆菌携带通过将外源基因导入所需的表达载体，其在培养基中培养在0.3％的浓度包含糖成分由大肠杆菌作为碳源利用的构成的杂交质粒或更多所以没有的表达 说，抑制所需外源基因。 该大肠杆菌，其在培养基中培养，其中在第一过程保持在0.3％以上的糖浓度以抑制外源基因，并支持足够的细胞生长，随后在小于0.3％的第二过程以释放 抑制允许有效生产外源基因产物，从而导致在最终培养外源基因产物的高浓度。