发明公开
- 专利标题: Novel plasmids
- 专利标题(中): Plasmide。
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申请号: EP88103172.8申请日: 1988-03-02
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公开(公告)号: EP0281104A1公开(公告)日: 1988-09-07
- 发明人: Shimazu, Mitsunobu , Terasawa, Masato , Yukawa, Hideaki
- 申请人: RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 申请人地址: 4-11, Shiba 3-chome Minato-ku Tokyo JP
- 专利权人: RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 当前专利权人: RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL
- 当前专利权人地址: 4-11, Shiba 3-chome Minato-ku Tokyo JP
- 代理机构: Kraus, Walter, Dr.
- 优先权: JP46856/87 19870303; JP290484/87 19871119
- 主分类号: C12N15/60
- IPC分类号: C12N15/60 ; C12N15/68 ; C12N1/20 ; C12P13/22
摘要:
A plasmid consisting essentially of
(a) a DNA fragment containing tryptophanase structural genes,
(b) a DNA fragment containing a tryptophan promoter and operator which control the expression of the tryptophanase structural genes,
(c) a DNA fragment containing genes governing the automonous replication system of a Col E1-type plasmid, and
(d) a DNA fragment containing genes derived from F-plasmid and governing its partition system.
Microorganisms transformed with the said plasmid were useful for the production of L-tryptophan having excellent producibility of tryptophanase.
(a) a DNA fragment containing tryptophanase structural genes,
(b) a DNA fragment containing a tryptophan promoter and operator which control the expression of the tryptophanase structural genes,
(c) a DNA fragment containing genes governing the automonous replication system of a Col E1-type plasmid, and
(d) a DNA fragment containing genes derived from F-plasmid and governing its partition system.
Microorganisms transformed with the said plasmid were useful for the production of L-tryptophan having excellent producibility of tryptophanase.
公开/授权文献
- EP0281104B1 Novel plasmids 公开/授权日:1993-07-21
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