利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12 条记录 (耗时 0.014 秒)

    Cultivation of transformed microorganisms
    5.
    发明公开
    Cultivation of transformed microorganisms 失效
    Züchtungvon transformierten Mikroorganismen。

    公开(公告)号：EP0397097A1

    公开(公告)日：1990-11-14

    申请号：EP90108609.0

    申请日：1990-05-08

    申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

    发明人： Terasawa, Masato ,  Yamagata, Hisashi ,  Yukawa, Hideaki ,  Fukushima, Makiko ,  Kurusu, Yasurou

    IPC分类号： C12N15/60 ,  C12P13/22 ,  C12N9/88 ,  C12N1/21

    CPC分类号： C12N9/88 ,  C12P13/227 ,  Y10S435/849

    摘要： A process for cultivating a microorganism transformed with a recombinant plasmid at least contain­ing (a) a DNA fragment containing a promoter and a regu­lator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment con­taining a desired structural gene which can express by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3 %, and thereby allowing the desired structural gene to express in the microorganism.

    摘要翻译： 培养用重组质粒转化的微生物的方法，所述重组质粒至少含有（a）含有启动子的DNA片段和位于启动子下游的色氨酸酶操纵子（tna）中的调节基因tnaC的方法，和（b）含有 可以由启动子表达的所需结构基因，其包括在连续或间歇地添加作为碳源的葡萄糖的同时在培养基中培养转化的微生物，使得葡萄糖的浓度保持在0.01〜0.3％的范围内，从而允许 在微生物中表达的所需结构基因。

    Novel plasmids
    6.
    发明公开
    Novel plasmids 失效
    Plasmide。

    公开(公告)号：EP0281104A1

    公开(公告)日：1988-09-07

    申请号：EP88103172.8

    申请日：1988-03-02

    申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

    发明人： Shimazu, Mitsunobu ,  Terasawa, Masato ,  Yukawa, Hideaki

    IPC分类号： C12N15/60 ,  C12N15/68 ,  C12N1/20 ,  C12P13/22

    CPC分类号： C12N15/68 ,  C12N9/88 ,  C12N15/74 ,  C12P13/227

    摘要： A plasmid consisting essentially of

    (a) a DNA fragment containing tryptophanase structural genes,
    (b) a DNA fragment containing a tryptophan promoter and operator which control the expression of the tryptophanase structural genes,
    (c) a DNA fragment containing genes governing the automonous replication system of a Col E1-type plasmid, and
    (d) a DNA fragment containing genes derived from F-plasmid and governing its partition system.
    Microorganisms transformed with the said plasmid were useful for the production of L-tryptophan having excellent producibility of tryptophanase.

    摘要翻译： 一种基本上由（a）含有色氨酸酶结构基因的DNA片段组成的质粒，（b）含有色氨酸启动子的DNA片段和控制色氨酸酶结构基因表达的操纵子，（c）含有控制自动复制基因的DNA片段 Col E1型质粒的系统，（d）含有来自F-质粒的基因的DNA片段，并控制其分配系统。 用所述质粒转化的微生物可用于生产具有优异色氨酸酶生产能力的L-色氨酸。

    Novel plasmids
    7.
    发明公开
    Novel plasmids 失效
    Plasmide。

    公开(公告)号：EP0180192A1

    公开(公告)日：1986-05-07

    申请号：EP85113730.7

    申请日：1985-10-29

    申请人： RESEARCH ASSOCIATION FOR UTILIZATION OF LIGHT OIL

    发明人： Yukawa, Hideaki ,  Terasawa, Masato ,  Kurusu, Yasuro ,  Shimazu, Mitsunobu

    IPC分类号： C12N15/60 ,  C12N15/68 ,  C12N15/69 ,  C12N1/20 ,  C12P13/22

    CPC分类号： C12N15/68 ,  C12N9/88 ,  C12N15/69 ,  C12P13/227

    摘要： A novel plasmid which can be autonomously replicated within the cells of Escherichia coli, said plasmid comprising a DNA fragment containing genes which govern the biosynthesis of tryptophan synthase, a DNA fragment containing genes which are derived from F-plasmid and govern its partition system and a DNA fragment containing genes which are derived from a Col El-type plasmid and govern its autonomous replication. A host organism transformed with the plasmid is useful for industrial production of tryptophan.

    摘要翻译： 一种能够在大肠杆菌细胞内自主复制的新型质粒，所述质粒包含含有控制色氨酸合成酶生物合成的基因的DNA片段，含有源于F-质粒的基因并控制其分配系统的DNA片段和 含有衍生自Col El型质粒并控制其自主复制的基因的DNA片段。 用质粒转化的宿主生物体可用于工业生产色氨酸。