发明公开
EP0327363A2 Separation of large DNA molecules in alternating asymmetric electric fields
失效
Trennung von grossen DNA-Molekülenin asymmetricrischen elektrischen Wechselfeldern。
- 专利标题： Separation of large DNA molecules in alternating asymmetric electric fields
- 专利标题（中）： Trennung von grossen DNA-Molekülenin asymmetricrischen elektrischen Wechselfeldern。
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申请号： EP89301010.8申请日： 1989-02-02
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公开(公告)号： EP0327363A2公开(公告)日： 1989-08-09
- 发明人: Stamato, Thomas D. , Denko, Nicholas
- 申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY
- 申请人地址： 36th & Spruce Streets Philadelphia, PA 19104 US
- 专利权人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY
- 当前专利权人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY
- 当前专利权人地址： 36th & Spruce Streets Philadelphia, PA 19104 US
- 代理机构： Hale, Stephen Geoffrey
- 优先权： US151651 19880203
- 主分类号： G01N27/26
- IPC分类号： G01N27/26
摘要：
DNA molecules are separated by a gel electrophoresis method which employes alternating higher and lower strength, electric fields applied in opposite directions to a gel matrix containing DNA. The higher strength field is maintained for a shorter interval than the lower strength field, and the ratio of the product of the lower strength field and its pulse interval to the product of the higher strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the lower strength field.
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