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1.发明公开Separation of large DNA molecules in alternating asymmetric electric fields 失效Trennung von grossen DNA-Molekülenin asymmetricrischen elektrischen Wechselfeldern。
公开(公告)号：EP0327363A2
公开(公告)日：1989-08-09
申请号：EP89301010.8
申请日：1989-02-02
IPC分类号： G01N27/26
CPC分类号： G01N27/44773
摘要： DNA molecules are separated by a gel electrophoresis method which employes alternating higher and lower strength, electric fields applied in opposite directions to a gel matrix containing DNA. The higher strength field is maintained for a shorter interval than the lower strength field, and the ratio of the product of the lower strength field and its pulse interval to the product of the higher strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the lower strength field.
摘要翻译： 通过凝胶电泳法分离DNA分子，该方法采用相对于含有DNA的凝胶基质相反方向施加交替的较高和较低强度的电场。 较高强度场保持较低强度场的较短的间隔，较低强度场与其脉冲间隔的乘积与高强度场与其脉冲间隔的乘积之比大于4：1， 足以产生DNA分子在较低强度场的方向上的净迁移。
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2.发明公开
公开(公告)号：EP0327363A3
公开(公告)日：1990-12-05
申请号：EP89301010.8
申请日：1989-02-02
IPC分类号： G01N27/26
CPC分类号： G01N27/44773
摘要： DNA molecules are separated by a gel electrophoresis method which employes alternating higher and lower strength, electric fields applied in opposite directions to a gel matrix containing DNA. The higher strength field is maintained for a shorter interval than the lower strength field, and the ratio of the product of the lower strength field and its pulse interval to the product of the higher strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the lower strength field.
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