发明公开
EP0354850A3 Preparation of immobilized lipases and their use in the synthesis of glycerides
失效
固定化饲料的制备及其在甘草酸合成中的应用
- 专利标题: Preparation of immobilized lipases and their use in the synthesis of glycerides
- 专利标题(中): 固定化饲料的制备及其在甘草酸合成中的应用
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申请号: EP89402261.5申请日: 1989-08-10
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公开(公告)号: EP0354850A3公开(公告)日: 1990-09-19
- 发明人: Ergan, Françoise , Trani, Michael , Andre, Gérald
- 申请人: HER MAJESTY IN RIGHT OF CANADA AS REPRESENTED BY THE NATIONAL RESEARCH COUNCIL OF CANADA
- 申请人地址: Montreal Road Ottawa Ontario K1A 0R6 CA
- 专利权人: HER MAJESTY IN RIGHT OF CANADA AS REPRESENTED BY THE NATIONAL RESEARCH COUNCIL OF CANADA
- 当前专利权人: HER MAJESTY IN RIGHT OF CANADA AS REPRESENTED BY THE NATIONAL RESEARCH COUNCIL OF CANADA
- 当前专利权人地址: Montreal Road Ottawa Ontario K1A 0R6 CA
- 代理机构: Ahner, Francis
- 优先权: US231221 19880811
- 主分类号: C12N11/02
- IPC分类号: C12N11/02 ; C12N9/16 ; C12P7/00
摘要:
A dry porous macromolecular matrix comprising a cross-linked mixture of 69.9 to 86.6% by weight of an inert protein and 0.4 to 10.2% by weight of a lipase enzyme and a cross-linking agent, the amount of cross-linking agent being about 11.7% to 23% by weight. Also within the scope of the present invention is a process for preparing the dry porous macromolecular matrix. This process comprises reacting the lipase enzyme, the inert protein and the cross-linking agent with a buffer solution, freezing the resulting reaction mixture at a temperature ranging between -20°C and -195°C, allowing the frozen mixture to thaw to a temperature ranging from 4°C to 25°C, and rinsing and drying the resulting product at room temperature.
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