发明授权
- 专利标题: PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION
- 专利标题(中): 通过同源重组蛋白的生产
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申请号: EP91900640.3申请日: 1990-11-06
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公开(公告)号: EP0452484B2公开(公告)日: 2004-07-28
- 发明人: SKOULTCHI, Arthur, I.
- 申请人: CELL GENESYS, INC.
- 申请人地址: 322 Lakeside Drive Foster City, CA 94404 US
- 专利权人: CELL GENESYS, INC.
- 当前专利权人: CELL GENESYS, INC.
- 当前专利权人地址: 322 Lakeside Drive Foster City, CA 94404 US
- 代理机构: Hallybone, Huw George
- 优先权: US432069 19891106
- 国际公布: WO1991006667 19910516
- 主分类号: C12P21/06
- IPC分类号: C12P21/06 ; C12P21/02 ; C12N15/00
摘要:
Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.
公开/授权文献
- EP0452484B1 PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION 公开/授权日:1996-06-19
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