发明公开
- 专利标题： Smooth muscle mitogen
- 专利标题（中）： 平滑肌肉MITOGEN
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申请号： EP91118120.4申请日： 1991-10-24
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公开(公告)号： EP0482623A3公开(公告)日： 1993-01-13
- 发明人: Folkman, Moses Judah , Shing, Yuen
- 申请人： CHILDREN'S MEDICAL CENTER CORPORATION
- 申请人地址： 55 Shattuck Street Boston Massachusetts 02115 US
- 专利权人： CHILDREN'S MEDICAL CENTER CORPORATION
- 当前专利权人： CHILDREN'S MEDICAL CENTER CORPORATION
- 当前专利权人地址： 55 Shattuck Street Boston Massachusetts 02115 US
- 代理机构： von Kreisler, Alek, Dipl.-Chem.
- 优先权： US604778 19901026; US776354 19910926
- 主分类号： C07K14/475
- IPC分类号： C07K14/475
摘要：
A novel growth factor (BTC-CG) was purified from the conditioned medium of pancreatic beta tumor cells initially derived from transgenic mice (RIP1-Tag2). The purification scheme included BioRex 70 chromatography, phenyl-Sepharose chromatography, TSL-GEL heparin FPLC and C4 reverse phase HPLC. The peptide also stimulated proliferation of bovine smooth muscle cells. It was not inactivated by boiling, by 10 mM dithiothreitol or by exposure to 1 M acetic acid. Biological activity of BTC-GF was recovered from a single band of protein which had a molecular weight of 32,000 on SDS-PAGE. The partial N-terminal amino acid sequence of this protein from BTC-3 and BTC-J10 cells was determined with an ABI 470A protein sequencer to be:
公开/授权文献
- EP0482623B1 Smooth muscle mitogen 公开/授权日：1995-05-10
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