发明公开
EP0645628A1 Immunological detection method of pyrethroid compound, haptenic compound, intermediate for the haptenic compound, immunogen and antibody
失效
对于拟除虫菊酯化合物的免疫学测定方法; 半抗原，对于半抗原化合物，免疫原和抗体的中间体。
- 专利标题： Immunological detection method of pyrethroid compound, haptenic compound, intermediate for the haptenic compound, immunogen and antibody
- 专利标题（中）： 对于拟除虫菊酯化合物的免疫学测定方法; 半抗原，对于半抗原化合物，免疫原和抗体的中间体。
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申请号： EP94115302.5申请日： 1994-09-28
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公开(公告)号： EP0645628A1公开(公告)日： 1995-03-29
- 发明人: Ohkawa, Hideo , Kitajima, Koji , Kodaka, Kenji , Fumoto, Yasunori , Kumeta, Takako
- 申请人： MITSUI TOATSU CHEMICALS, Inc.
- 申请人地址： 2-5 Kasumigaseki 3-chome Chiyoda-Ku Tokyo 100 JP
- 专利权人： MITSUI TOATSU CHEMICALS, Inc.
- 当前专利权人： MITSUI TOATSU CHEMICALS, Inc.
- 当前专利权人地址： 2-5 Kasumigaseki 3-chome Chiyoda-Ku Tokyo 100 JP
- 代理机构： Zumstein, Fritz, Dr.
- 优先权： JP241037/93 19930928
- 主分类号： G01N33/53
- IPC分类号： G01N33/53 ; G01N33/535 ; G01N33/541
摘要：
Described is a detection method of a pyrethroid compound which comprises providing a first antibody against the pyrethroid compound, said first antibody having been obtained by immunizing a responder with an immunogen which is a first conjugate between a first carrier and a first haptenic compound represented by the following formula (1) or (2):
mixing the first antibody with the sample solution,
causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; and
reacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith.
This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.
mixing the first antibody with the sample solution,
causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; and
reacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith.
This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.
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