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1.发明公开Immunological detection method of pyrethroid compound, haptenic compound, intermediate for the haptenic compound, immunogen and antibody 失效对于拟除虫菊酯化合物的免疫学测定方法; 半抗原，对于半抗原化合物，免疫原和抗体的中间体。
公开(公告)号：EP0645628A1
公开(公告)日：1995-03-29
申请号：EP94115302.5
申请日：1994-09-28
IPC分类号： G01N33/53 , G01N33/535 , G01N33/541
CPC分类号： C07C43/295 , C07C43/315 , C07K16/44 , G01N33/5308
摘要： Described is a detection method of a pyrethroid compound which comprises providing a first antibody against the pyrethroid compound, said first antibody having been obtained by immunizing a responder with an immunogen which is a first conjugate between a first carrier and a first haptenic compound represented by the following formula (1) or (2):
mixing the first antibody with the sample solution,
causing any unreacted portion of the first antibody to be bound on a second conjugate as a coated antigen, said second conjugate being formed of a second carrier and a second haptenic compound and having been coated on a surface of a solid phase carrier, whereby an antigen-antibody conjugate is obtained; and
reacting the antigen-antibody conjugate with a second antibody against the first antibody, said second antibody having a labelled enzyme linked therewith.
This invention has made it possible to conduct easy, economical and quick analysis of a pyrethroid compound contained in our surroundings such as water or soil, plants or foods.摘要翻译： 所描述的是，其包括：提供第一抗体对抗拟除虫菊酯化合物拟除虫菊酯化合物的检测方法，所述通过在免疫原性免疫应答与所有其为第一载波和第一半抗原化合物所代表之间的第一缀合物已经被获得的第一抗体 下式（1）或（2）：
混合第一抗体与样品溶液，导致要在第二共轭作为包被抗原结合的第一抗体的任何未反应的部分，所述第二共轭形成的第二的 载波和第二半抗原化合物与已涂覆的固相载体的表面，由此在抗原 - 抗体接合物，得到具有上; 反应并与针对第一抗体的第二抗体的抗原 - 抗体接合物，所述具有连接的有标记的酶的第二抗体。 本发明使得有可能进行包含在我们的周围拟除虫菊酯化合物的方便，经济快速分析：例如水或土壤，植物或食物。
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