发明公开
EP0755447A1 PROTEINES RECOMBINANTES DE L'HEMAGGLUTININE FILAMENTEUSE DE $i(BORDETELLA), NOTAMMENT $i(BORDETELLA PERTUSSIS), PROCEDE POUR LEUR PRODUCTION ET LEURS APPLICATIONS A LA PRODUCTION DE PROTEINES ETRANGERES OU DE PRINCIPES ACTIFS VACCINANTS
失效
百日咳，尤其是百日咳百日咳，方法与VERWENDUNGZUR重组PROTEUINE丝状HAEMAGLUTININS产生杂质蛋白或疫苗
- 专利标题： PROTEINES RECOMBINANTES DE L'HEMAGGLUTININE FILAMENTEUSE DE $i(BORDETELLA), NOTAMMENT $i(BORDETELLA PERTUSSIS), PROCEDE POUR LEUR PRODUCTION ET LEURS APPLICATIONS A LA PRODUCTION DE PROTEINES ETRANGERES OU DE PRINCIPES ACTIFS VACCINANTS
- 专利标题（英）： RECOMBINANT PROTEINS OF FILAMENTOUS HAEMAGGLUTININ OF $i(BORDETELLA), PARTICULARLY $i(BORDETELLA PERTUSSIS), METHOD FOR PRODUCING SAME, AND USES THEREOF FOR PRODUCING FOREIGN PROTEINS OR VACCINATING ACTIVE PRINCIPLES
- 专利标题（中）： 百日咳，尤其是百日咳百日咳，方法与VERWENDUNGZUR重组PROTEUINE丝状HAEMAGLUTININS产生杂质蛋白或疫苗
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申请号： EP95918030.0申请日： 1995-04-19
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公开(公告)号： EP0755447A1公开(公告)日： 1997-01-29
- 发明人: LOCHT, Camille , RENAULD, Geneviève , CAPRON, André , RIVEAU, Gilles , MENOZZI, Franco , JACOB-DUBUISSON, Françoise
- 申请人： INSTITUT PASTEUR , INSTITUT PASTEUR DE LILLE , INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
- 申请人地址： 25-28, rue du Docteur Roux F-75724 Paris Cédex 15 FR
- 专利权人： INSTITUT PASTEUR,INSTITUT PASTEUR DE LILLE,INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
- 当前专利权人： INSTITUT PASTEUR,INSTITUT PASTEUR DE LILLE,INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
- 当前专利权人地址： 25-28, rue du Docteur Roux F-75724 Paris Cédex 15 FR
- 代理机构： Gutmann, Ernest, et al
- 优先权： FR19940004661 19940419
- 国际公布： WO1995028486 19951026
- 主分类号： C07K14
- IPC分类号： C07K14 ; C12N15
摘要：
A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the same reading frame to a sequence (2) located upstream from the first sequence. Said sequence (2) codes for at least a part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein which contains the interaction site of Fha and heparin, and which, when it is itself subjected on its own to the control of a promoter recognised by the cell polymerases of B. Pertussis, and inserted into a B. Pertussis cell culture, is expressed in said culture under the control of said promoter and excreted into the cell culture medium. The invention uses both the abilities of Bordetella, and particularly B. Pertussis, which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from those of the Bordetella synthesising same.
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