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1.发明公开PROTEINES RECOMBINANTES DE L'HEMAGGLUTININE FILAMENTEUSE DE $i(BORDETELLA), NOTAMMENT $i(BORDETELLA PERTUSSIS), PROCEDE POUR LEUR PRODUCTION ET LEURS APPLICATIONS A LA PRODUCTION DE PROTEINES ETRANGERES OU DE PRINCIPES ACTIFS VACCINANTS 失效百日咳，尤其是百日咳百日咳，方法与VERWENDUNGZUR重组PROTEUINE丝状HAEMAGLUTININS产生杂质蛋白或疫苗
公开(公告)号：EP0755447A1
公开(公告)日：1997-01-29
申请号：EP95918030.0
申请日：1995-04-19
申请人： INSTITUT PASTEUR , INSTITUT PASTEUR DE LILLE , INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
发明人： LOCHT, Camille , RENAULD, Geneviève , CAPRON, André , RIVEAU, Gilles , MENOZZI, Franco , JACOB-DUBUISSON, Françoise
CPC分类号： C07K14/235 , C07K2319/00
摘要： A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the same reading frame to a sequence (2) located upstream from the first sequence. Said sequence (2) codes for at least a part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein which contains the interaction site of Fha and heparin, and which, when it is itself subjected on its own to the control of a promoter recognised by the cell polymerases of B. Pertussis, and inserted into a B. Pertussis cell culture, is expressed in said culture under the control of said promoter and excreted into the cell culture medium. The invention uses both the abilities of Bordetella, and particularly B. Pertussis, which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from those of the Bordetella synthesising same.
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2.发明授权
公开(公告)号：EP0755447B1
公开(公告)日：2003-07-02
申请号：EP95918030.8
申请日：1995-04-19
发明人： LOCHT, Camille , RENAULD, Geneviève , CAPRON, André , RIVEAU, Gilles , MENOZZI, Franco , JACOB-DUBUISSON, Françoise
IPC分类号： C12N15/31 , C12N15/62 , C07K14/235 , C12N1/21 , A61K39/10
CPC分类号： C07K14/235 , C07K2319/00
摘要： A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the same reading frame to a sequence (2) located upstream from the first sequence. Said sequence (2) codes for at least a part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein which contains the interaction site of Fha and heparin, and which, when it is itself subjected on its own to the control of a promoter recognised by the cell polymerases of B. Pertussis, and inserted into a B. Pertussis cell culture, is expressed in said culture under the control of said promoter and excreted into the cell culture medium. The invention uses both the abilities of Bordetella, and particularly B. Pertussis, which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from those of the Bordetella synthesising same.
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