发明授权
- 专利标题: Methods of production of Herpes Simplex Virus 1 or 2 protease
- 专利标题(中): 制作方法为单纯疱疹病毒1种或2蛋白酶
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申请号: EP95927255.0申请日: 1995-07-17
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公开(公告)号: EP0777727B1公开(公告)日: 2003-02-26
- 发明人: DARKE, Paul L. , HALL, Dawn L. , KUO, Lawrence C.
- 申请人: Merck & Co., Inc. (a New Jersey corp.)
- 申请人地址: 126 East Lincoln Avenue Rahway, N.J. 07065 US
- 专利权人: Merck & Co., Inc. (a New Jersey corp.)
- 当前专利权人: Merck & Co., Inc. (a New Jersey corp.)
- 当前专利权人地址: 126 East Lincoln Avenue Rahway, N.J. 07065 US
- 代理机构: Hiscock, Ian James
- 优先权: US279754 19940721
- 国际公布: WO96003498 19960208
- 主分类号: C12N9/50
- IPC分类号: C12N9/50
摘要:
A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic cleavage occurs, resulting in mature protease.
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