发明公开
- 专利标题： IMPROVED ADENOVIRUS AND METHODS OF USE THEREOF
- 专利标题（中）： 重组腺病毒的方法及其用途
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申请号： EP95942839.0申请日： 1995-10-27
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公开(公告)号： EP0787200A2公开(公告)日： 1997-08-06
- 发明人: WILSON, James, M. , FISHER, Krishna, J. , CHEN, Shu-Jen , WEITZMAN, Matthew
- 申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- 申请人地址： Technology Transfer Center, 133 South 36th Street, Suite 419 Philadelphia, PA 19104-3246 US
- 专利权人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- 当前专利权人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
- 当前专利权人地址： Technology Transfer Center, 133 South 36th Street, Suite 419 Philadelphia, PA 19104-3246 US
- 代理机构： Hale, Stephen Geoffrey, et al
- 优先权： US19940331381 19941028
- 国际公布： WO1996013597 19960509
- 主分类号： C12N15
- IPC分类号： C12N15 ; A61K35 ; A61K48 ; C12N7
摘要：
A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.
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