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公开(公告)号：EP0787200A2
公开(公告)日：1997-08-06
申请号：EP95942839.0
申请日：1995-10-27
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2710/10343 , C12N2810/6018 , C12N2840/44
摘要： A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.
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2.发明授权IMPROVED ADENOVIRUS AND METHODS OF USE THEREOF 失效REKOMBINANTER ADENOVIRUS UND METHODEN ZU DESSEN VERWENDUNG
公开(公告)号：EP0787200B1
公开(公告)日：2005-04-20
申请号：EP95942839.2
申请日：1995-10-27
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2710/10343 , C12N2810/6018 , C12N2840/44
摘要： A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.
摘要翻译： 提供重组腺病毒及其制备方法，该方法利用包含腺病毒DNA序列的重组穿梭载体，用于在不存在编码病毒基因的序列和选择的小基因的情况下用于复制和病毒粒子壳化所需的5'和3'顺式元件 和包含足够的用于生产性病毒感染所必需的腺病毒基因序列的辅助性腺病毒。 帮助基因通过修饰其5'包装序列而被削弱，这有助于从辅助病毒纯化病毒颗粒。
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