There is disclosed an endoscopic ligation kit which is capable of successively effecting ligations safely and positively. This kit includes an inner cylinder, an outer cylinder having at least two bamboo shoot-like steps formed on an outer periphery of its distal end portion, and a slide cylinder provided between the inner cylinder and the outer cylinder. The outer cylinder has a notch extending from its distal end to its maximum outer diameter portion. On the other hand, the slide cylinder has at its distal end portion a projection which conforms in size to the notch, and has steps, and the projection is fitted in the notch. O-rings are mounted respectively on the outer periphery of the distal end portion of the inner cylinder and the step portions of the outer cylinder. When a fluid is fed into an annular hermetic space, formed at a rear side of the slide cylinder, to move the slide cylinder forwardly, the O-ring, mounted on the outer periphery of the distal end portion of the inner cylinder, is disengaged therefrom, and also the O-rings, mounted respectively on the step portions of the outer cylinder, are moved to the forwardly-adjacent step portions, respectively.