发明授权
- 专利标题： DNA POLYMORPHISM IDENTITY DETERMINATION USING FLOW CYTOMETRY
- 专利标题（中）： 通过流式细胞术DNA多态性鉴定
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申请号： EP98955222.9申请日： 1998-10-28
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公开(公告)号： EP1040202B1公开(公告)日： 2008-12-31
- 发明人: NOLAN, John, P. , WHITE, P., Scott , CAI, Hong
- 申请人： Los Alamos National Security, LLC
- 申请人地址： Los Alamos National Laboratory LC/IP, MS A187 Los Alamos, NM 87545 US
- 专利权人： Los Alamos National Security, LLC
- 当前专利权人： Los Alamos National Security, LLC
- 当前专利权人地址： Los Alamos National Laboratory LC/IP, MS A187 Los Alamos, NM 87545 US
- 代理机构： Capasso, Olga
- 优先权： US63685 19971028
- 国际公布： WO1999022030 19990506
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/02 ; C07H21/04 ; C12N15/00
摘要：
Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.
公开/授权文献
- EP1040202B9 DNA POLYMORPHISM IDENTITY DETERMINATION USING FLOW CYTOMETRY 公开/授权日：2009-09-23
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