发明公开
- 专利标题： PROCEDE DE SEPARATION ET DE CARACTERISATION DES FONCTIONS POTENTIELLEMENT PRESENTES DANS UN ECHANTILLON BIOLOGIQUE CONTENANT DES ACIDES NUCLEIQUES
- 专利标题（英）： Method for separating and characterising functions potentially present in a biological sample containing nucleic acids
- 专利标题（中）： 进行隔离势函数的表征方法对生物样品的基础上，它包含NUCLEIC
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申请号： EP99936724.6申请日： 1999-08-11
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公开(公告)号： EP1104489A1公开(公告)日： 2001-06-06
- 发明人: DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice
- 申请人： Proteus S.A.
- 申请人地址： Parc Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
- 专利权人： Proteus S.A.
- 当前专利权人： Proteus S.A.
- 当前专利权人地址： Parc Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
- 代理机构： Breese, Pierre
- 优先权： FR9810337 19980812
- 国际公布： WO0009747 20000224
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/00 ; C12N15/63
摘要：
The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.
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