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1.发明授权METHODE DE DIAGNOSTIC BASEE SUR LA FONCTION D'UNE PROTEINE PRODUITE DANS UN SYSTEME ACELLULAIRE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES 有权诊断方法基于的蛋白在细胞中-FREE环境中产生的功能，ON NUCLEIC的样品的基础上
公开(公告)号：EP1137801B1
公开(公告)日：2004-02-18
申请号：EP99957373.6
申请日：1999-12-08
申请人： Proteus S.A.
发明人： BLOCH, Jean-François , DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice , DAUTEL, Sandrine
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6897 , C12Q2525/143 , C12Q2521/119
摘要： The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
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2.发明公开METHODE DE DTECTION ET/OU DE QUANTIFICATION D'UNE FONCTION CONNUE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES 有权诊断方法基于的蛋白在细胞中-FREE环境中产生的功能，ON NUCLEIC的样品的基础上
公开(公告)号：EP1137801A1
公开(公告)日：2001-10-04
申请号：EP99957373.6
申请日：1999-12-08
申请人： Proteus S.A.
发明人： BLOCH, Jean-François , DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice , DAUTEL, Sandrine
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6897 , C12Q2525/143 , C12Q2521/119
摘要： The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
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3.发明授权
公开(公告)号：EP1104489B1
公开(公告)日：2007-10-31
申请号：EP99936724.6
申请日：1999-08-11
申请人： Proteus S.A.
CPC分类号： C12Q1/68
摘要： The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.
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4.发明授权PROCEDE D'OBTENTION IN VITRO DE SEQUENCES POLYNUCLEOTIDIQUES RECOMBINEES, BANQUES DE SEQUENCES ET SEQUENCES AINSI OBTENUES 有权工艺用于在重组的碱基序列，SO HERGESTELTE格涅·班克斯和序列的体外
公开(公告)号：EP1104457B1
公开(公告)日：2005-11-09
申请号：EP99936725.3
申请日：1999-08-11
申请人： Proteus S.A.
CPC分类号： C12N15/1027 , C07K14/245
摘要： The invention concerns a method for producing in vitro recombined polynucleotide sequences from a bank of polynucleotide sequences, characterised in that it comprises the following steps: fragmenting an initial bank of double-strand polynucleotide sequences; (b) denaturation of the fragments resulting from step (a) optionally in the presence of one or several assembling matrix or matrices; (c) hybridising said fragments with one or several of the assembling matrix or matrices if the latter is/are not present in step (b); (d) ligation of said fragments; (e) optionally cloning said recombined polynucleotide sequences having advantageous properties with respect to one or several reference sequences.
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5.发明授权METHODE DE DIAGNOSTIC BASEE SUR LA FONCTION D'UNE PROTEINE PRODUITE DANS UN SYSTEME ACELLULAIRE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES 有权诊断方法基于的蛋白在细胞中-FREE环境中产生的功能，ON NUCLEIC的样品的基础上
公开(公告)号：EP1137801B9
公开(公告)日：2004-10-06
申请号：EP99957373.6
申请日：1999-12-08
申请人： Proteus S.A.
发明人： BLOCH, Jean-François , DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice , DAUTEL, Sandrine
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6897 , C12Q2525/143 , C12Q2521/119
摘要： The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
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6.发明公开
公开(公告)号：EP1137802A1
公开(公告)日：2001-10-04
申请号：EP99957374.4
申请日：1999-12-08
申请人： Proteus S.A.
发明人： BLOCH, Jean-François , DAUTEL, Sandrine , DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice
IPC分类号： C12Q1/68
CPC分类号： G01N33/68 , C12Q1/6897 , G01N33/6845 , G01N2500/00
摘要： The invention concerns a method for determining the activity in vitro of a substance using a functional test, characterised in that it consists in detecting and/or measuring the variation of a known function corresponding to one or several proteins produced in vitro in the presence and in the absence of said substance or to the substance in the presence or in the absence of one or several proteins produced in vitro.
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7.发明公开
公开(公告)号：EP1104489A1
公开(公告)日：2001-06-06
申请号：EP99936724.6
申请日：1999-08-11
申请人： Proteus S.A.
CPC分类号： C12Q1/68
摘要： The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.
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8.发明公开PROCEDE D'OBTENTION IN VITRO DE SEQUENCES POLYNUCLEOTIDIQUES RECOMBINEES, BANQUES DE SEQUENCES ET SEQUENCES AINSI OBTENUES 有权工艺用于在重组的碱基序列，SO HERGESTELTE格涅·班克斯和序列的体外
公开(公告)号：EP1104457A1
公开(公告)日：2001-06-06
申请号：EP99936725.3
申请日：1999-08-11
申请人： Proteus S.A.
CPC分类号： C12N15/1027 , C07K14/245
摘要： The invention concerns a method for producing in vitro recombined polynucleotide sequences from a bank of polynucleotide sequences, characterised in that it comprises the following steps: fragmenting an initial bank of double-strand polynucleotide sequences; (b) denaturation of the fragments resulting from step (a) optionally in the presence of one or several assembling matrix or matrices; (c) hybridising said fragments with one or several of the assembling matrix or matrices if the latter is/are not present in step (b); (d) ligation of said fragments; (e) optionally cloning said recombined polynucleotide sequences having advantageous properties with respect to one or several reference sequences.
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9.发明授权
公开(公告)号：EP1137802B1
公开(公告)日：2006-02-08
申请号：EP99957374.4
申请日：1999-12-08
申请人： Proteus S.A.
发明人： BLOCH, Jean-François , DAUTEL, Sandrine , DUPRET, Daniel , MASSON, Jean-Michel , LEFEVRE, Fabrice
IPC分类号： C12Q1/68
CPC分类号： G01N33/68 , C12Q1/6897 , G01N33/6845 , G01N2500/00
摘要： The invention concerns a method for determining the activity in vitro of a substance using a functional test, characterised in that it consists in detecting and/or measuring the variation of a known function corresponding to one or several proteins produced in vitro in the presence and in the absence of said substance or to the substance in the presence or in the absence of one or several proteins produced in vitro.
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