发明公开
EP1117841A1 METHOD FOR IDENTIFYING COMPOUNDS AFFECTING RNA/RNA BINDING PROTEIN INTERACTIONS
有权
对于RNA相互作用的化合物的检测和蛋白质的方法,结合RNA LEG河流
- 专利标题: METHOD FOR IDENTIFYING COMPOUNDS AFFECTING RNA/RNA BINDING PROTEIN INTERACTIONS
- 专利标题(中): 对于RNA相互作用的化合物的检测和蛋白质的方法,结合RNA LEG河流
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申请号: EP99970130.3申请日: 1999-09-16
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公开(公告)号: EP1117841A1公开(公告)日: 2001-07-25
- 发明人: GIORDANO, Tony , BEACH, Deborah, L. , TEMELES, Gretchen, L.
- 申请人: Message Pharmaceuticals, Inc.
- 申请人地址: 30 Spring Mill Drive Malvern, PA 19355 US
- 专利权人: Message Pharmaceuticals, Inc.
- 当前专利权人: Message Pharmaceuticals, Inc.
- 当前专利权人地址: 30 Spring Mill Drive Malvern, PA 19355 US
- 代理机构: Grund, Martin, Dr.
- 优先权: US165868 19981002
- 国际公布: WO0020637 20000413
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
Disclosed are nucleic acid sequences which, when present in an RNA molecule, bind to RNA binding proteins. These nucleic acid sequences are present in untranslated regions of certain mRNA. They can be used in assays to identify compounds that affect the interaction of RNA containing the nucleic acid sequence, such as the source mRNA, and RNA binding proteins. The disclosed nucleic acid sequences can also be used to identify RNA binding proteins that can interact with the sequences. An assay for identifying compounds that affect interaction of RNA containing one of the disclosed nucleic acid sequences and RNA binding proteins is also disclosed. The assay involves detecting interactions between RNA binding proteins and an RNA molecule containing one of the disclosed nucleic acid sequences in the presence of a test compound and in the absence of the test compound. A difference in the detected interaction in the presence and absence of the test compound indicates that the compound affects the interaction. The identified compounds can be used to affect the interaction of RNA binding proteins with mRNA containing the nucleic acid sequences or related sequences.
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