发明公开
EP1137801A1 METHODE DE DTECTION ET/OU DE QUANTIFICATION D'UNE FONCTION CONNUE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES 有权
诊断方法基于的蛋白在细胞中-FREE环境中产生的功能,ON NUCLEIC的样品的基础上

  • 专利标题: METHODE DE DTECTION ET/OU DE QUANTIFICATION D'UNE FONCTION CONNUE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES
  • 专利标题(英): Method for detecting and/or quantifying a known function from a nucleic acid sample
  • 专利标题(中): 诊断方法基于的蛋白在细胞中-FREE环境中产生的功能,ON NUCLEIC的样品的基础上
  • 申请号: EP99957373.6
    申请日: 1999-12-08
  • 公开(公告)号: EP1137801A1
    公开(公告)日: 2001-10-04
  • 发明人: BLOCH, Jean-FrançoisDUPRET, DanielMASSON, Jean-MichelLEFEVRE, FabriceDAUTEL, Sandrine
  • 申请人: Proteus S.A.
  • 申请人地址: Parc Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
  • 专利权人: Proteus S.A.
  • 当前专利权人: Proteus S.A.
  • 当前专利权人地址: Parc Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
  • 代理机构: Breese, Pierre
  • 优先权: FR9815487 19981208
  • 国际公布: WO0034512 20000615
  • 主分类号: C12Q1/68
  • IPC分类号: C12Q1/68
METHODE DE DTECTION ET/OU DE QUANTIFICATION D'UNE FONCTION CONNUE A PARTIR D'UN ECHANTILLON D'ACIDES NUCLEIQUES
摘要:
The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
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