发明公开
- 专利标题： SCREENING FOR INHIBITORS OF TERPENOIDS BIOSYNTHESIS
- 专利标题（中）： 筛选抑制剂萜类化合物生物合成
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申请号： EP99963515.4申请日： 1999-12-13
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公开(公告)号： EP1141379A1公开(公告)日： 2001-10-10
- 发明人: BACHER, Adelbert , ZENK, Meinhart, H. , KIS, Klaus , FELLERMEIER, Monika
- 申请人： Bacher, Adelbert , Zenk, Meinhart H.
- 申请人地址： Königbergerstrasse 74 85748 Garching DE
- 专利权人： Bacher, Adelbert,Zenk, Meinhart H.
- 当前专利权人： Bacher, Adelbert,Zenk, Meinhart H.
- 当前专利权人地址： Königbergerstrasse 74 85748 Garching DE
- 代理机构： Wächtershäuser, Günter, Prof. Dr.
- 优先权： DE19857619 19981214; DE19948616 19991008; DE19948615 19991008
- 国际公布： WO0036138 20000622
- 主分类号： C12Q1/02
- IPC分类号： C12Q1/02 ; G01N33/92 ; C07H13/00 ; C07H3/02 ; A01N61/00
摘要：
A method is described for high-sensitivity screening for the presence of inhibition of at least one enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants by incubating an aqueous suspension of plastids or plastid-bearing cells in the presence of a labelled biochemical procursor for generating terpenoids via said pathway and preferably in the presence of a combination of ATP and CTP or a source thereof with or without the addition of a prospective inhibitor and determining the production of labelled product or intermediate downstream from 1-deoxy-D-xylulose 5-phosphate or notably the release of tritiated water. Further, labelled biochemical precursors are described as well as means and methods of inhibition.
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