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公开(公告)号：EP1141379A1
公开(公告)日：2001-10-10
申请号：EP99963515.4
申请日：1999-12-13
CPC分类号： C07H13/00 , C07H3/02 , C12Q1/025 , G01N33/5097 , G01N33/92 , G01N2500/00
摘要： A method is described for high-sensitivity screening for the presence of inhibition of at least one enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants by incubating an aqueous suspension of plastids or plastid-bearing cells in the presence of a labelled biochemical procursor for generating terpenoids via said pathway and preferably in the presence of a combination of ATP and CTP or a source thereof with or without the addition of a prospective inhibitor and determining the production of labelled product or intermediate downstream from 1-deoxy-D-xylulose 5-phosphate or notably the release of tritiated water. Further, labelled biochemical precursors are described as well as means and methods of inhibition.
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公开(公告)号：EP1198575B1
公开(公告)日：2007-09-19
申请号：EP00949452.7
申请日：2000-08-03
发明人： BACHER, Adelbert , ZENK, Meinhart H. , EISENREICH, Wolfgang , FELLERMEIER, Monika , FISCHER, Markus , HECHT, Stefan , HERZ, Stefan , KIS, Klaus , LÜTTGEN, Holger , ROHDICH, Felix , SAGNER, Silvia , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibiors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.
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公开(公告)号：EP1287145B1
公开(公告)日：2008-11-26
申请号：EP01940547.1
申请日：2001-06-01
发明人： BACHER, Adelbert , ZENK, Meinhart, H. , ADAM, Petra , EISENREICH, Wolfgang , FELLERMEIER, Monika , HECHT, Stefan , ROHDICH, Felix , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
CPC分类号： C12N9/00 , C12N15/52 , C12N15/8274 , C12P9/00
摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.
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公开(公告)号：EP1287145A2
公开(公告)日：2003-03-05
申请号：EP01940547.1
申请日：2001-06-01
发明人： BACHER, Adelbert , ZENK, Meinhart, H. , ADAM, Petra , EISENREICH, Wolfgang , FELLERMEIER, Monika , HECHT, Stefan , ROHDICH, Felix , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
CPC分类号： C12N9/00 , C12N15/52 , C12N15/8274 , C12P9/00
摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.
摘要翻译： 本发明涉及2C甲基-D-赤藓糖醇2,4-环焦磷酸酯下游和异戊烯基焦磷酸或二甲基烯丙基焦磷酸上游的非甲羟戊酸类异戊二烯途径的酶和中间体。 这些用作这些酶抑制剂筛选方法的基础，以及鉴定其抑制剂抗性变体的方法。 其它公开内容涉及编码所述酶及其抑制剂抗性变体的DNA，含有所述DNA的载体，含有所述载体的细胞和含有含所述载体的细胞的植物种子。 本发明可用于抑制植物，细菌和原生动物中类异戊二烯的生物合成，赋予植物除草剂抗性，以及在农业中用含有除草剂抗性基因和有效量的 合适的除草剂。
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公开(公告)号：EP1198575A1
公开(公告)日：2002-04-24
申请号：EP00949452.7
申请日：2000-08-03
发明人： BACHER, Adelbert , ZENK, Meinhart , EISENREICH, Wolfgang , FELLERMEIER, Monika , FISCHER, Markus , HECHT, Stefan , HERZ, Stefan , KIS, Klaus , LÜTTGEN, Holger , ROHDICH, Felix , SAGNER, Silvia , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibiors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.
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