发明授权
EP1141403B1 DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT
有权
DNA寡核苷酸特异性根结线虫种类，DNA载体和宿主生物体，包括和用途一起还原
- 专利标题： DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT
- 专利标题（中）： DNA寡核苷酸特异性根结线虫种类，DNA载体和宿主生物体，包括和用途一起还原
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申请号： EP99964776.1申请日： 1999-12-28
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公开(公告)号： EP1141403B1公开(公告)日： 2002-10-09
- 发明人: ZIJLSTRA, Carolien
- 申请人： Plant Research International B.V.
- 申请人地址： Droevendaalsesteeg 1 6708 PB Wageningen NL
- 专利权人： Plant Research International B.V.
- 当前专利权人： Plant Research International B.V.
- 当前专利权人地址： Droevendaalsesteeg 1 6708 PB Wageningen NL
- 代理机构： Van kan, Johan Joseph Hubert, Ir.
- 优先权： NL1010917 19981230
- 国际公布： WO00040754 20000713
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention relates to a DNA oligonucleotide which is specific for Meloidogyne species, which DNA oligonucleotide has the following sequence: (a) a DNA sequence chosen from the group consisting of SEQ ID No. 1 to SEQ ID No. 20, or (b) a DNA sequence which under conditions of stringency hybridises with a sequence according to (a) and which is specific for Meloidogyne species. Furthermore, the invention relates to a DNA vector containing such a DNA oligonucleotide, as well as to a host cell containing such vector. The invention also relates to a process for determining the presence of a Meloidogyne species, according to which DNA is isolated from a sample to be analysed, and is subsequently subjected to a PCR reaction, using a DNA oligonucleotide according to the invention. Finally, the invention relates to a kit for determining the presence of a Meloidogyne species.
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