发明公开
EP1332216A1 VARIANTS OF SOLUBLE PYRROLOQUINOLINE QUINONE-DEPENDENT GLUCOSE DEHYDROGENASE
有权
可溶性PYRROLOQUINOLIN醌依赖性葡萄糖脱氢酶的变异研究
- 专利标题: VARIANTS OF SOLUBLE PYRROLOQUINOLINE QUINONE-DEPENDENT GLUCOSE DEHYDROGENASE
- 专利标题(中): 可溶性PYRROLOQUINOLIN醌依赖性葡萄糖脱氢酶的变异研究
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申请号: EP01988775.1申请日: 2001-10-20
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公开(公告)号: EP1332216A1公开(公告)日: 2003-08-06
- 发明人: KRATZSCH, Peter , SCHMUCK, Rainer , BUNK, Daniela , SHAO, Zhixin , THYM, Detlef , KNAPPE, Wolfgang-Reinhold
- 申请人: Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
- 申请人地址: Sandhofer Strasse 116 68305 Mannheim DE
- 专利权人: Roche Diagnostics GmbH,F. HOFFMANN-LA ROCHE AG
- 当前专利权人: Roche Diagnostics GmbH,F. HOFFMANN-LA ROCHE AG
- 当前专利权人地址: Sandhofer Strasse 116 68305 Mannheim DE
- 优先权: EP00123512 20001027; EP00127294 20001219
- 国际公布: WO02034919 20020502
- 主分类号: C12N15/53
- IPC分类号: C12N15/53 ; C12N9/04 ; C12Q1/32 ; C12Q1/54
摘要:
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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