发明公开
EP1381680A1 TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES 审中-公开
DIES交换,结扎为导向的多核苷酸的并不是偶然的洗牌方法

  • 专利标题: TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES
  • 专利标题(中): DIES交换,结扎为导向的多核苷酸的并不是偶然的洗牌方法
  • 申请号: EP02743543.7
    申请日: 2002-04-25
  • 公开(公告)号: EP1381680A1
    公开(公告)日: 2004-01-21
  • 发明人: DUPRET, DanielLEFEVRE, FabriceMASSON, Jean-Michel
  • 申请人: Proteus
  • 申请人地址: Parc Scientifique Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
  • 专利权人: Proteus
  • 当前专利权人: Proteus
  • 当前专利权人地址: Parc Scientifique Georges Besse,70, Allée Graham Bell 30000 Nîmes FR
  • 代理机构: Breese, Pierre
  • 优先权: US285998P 20010425; US840861 20010425; FR0105573 20010425
  • 国际公布: WO2002086121 20021031
  • 主分类号: C12N15/10
  • IPC分类号: C12N15/10 C12Q1/68
TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES
摘要:
Method of gene shuffling oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
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