发明公开
EP1382672A4 SMALL LIVER CELL-RICH COLONY, METHOD OF PREPARING THE SAME, METHOD OF MATURING THE SAME INTO LIVER TISSUE AND METHOD OF ESTIMATING DRUG FUNCTION USING THE MATURED SMALL LIVER CELL-RICH COLONY
审中-公开
TO小肝癌细胞内含有丰富COLONY，制作方法，炼制肝组织方法和评价药物物质的功能的方法，使用成熟的TO小肝癌细胞内含有丰富COLONY
- 专利标题： SMALL LIVER CELL-RICH COLONY, METHOD OF PREPARING THE SAME, METHOD OF MATURING THE SAME INTO LIVER TISSUE AND METHOD OF ESTIMATING DRUG FUNCTION USING THE MATURED SMALL LIVER CELL-RICH COLONY
- 专利标题（中）： TO小肝癌细胞内含有丰富COLONY，制作方法，炼制肝组织方法和评价药物物质的功能的方法，使用成熟的TO小肝癌细胞内含有丰富COLONY
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申请号： EP02718561申请日： 2002-04-12
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公开(公告)号： EP1382672A4公开(公告)日： 2004-06-30
- 发明人: MITAKA TOSHIHIRO , SUGIMOTO SHINICHI
- 申请人： HOKKAIDO TECH LICENSING OFFICE
- 专利权人： HOKKAIDO TECH LICENSING OFFICE
- 当前专利权人： HOKKAIDO TECH LICENSING OFFICE
- 优先权： JP2001125525 2001-04-24
- 主分类号： A61K35/12
- IPC分类号： A61K35/12 ; C12N5/071 ; G01N33/50 ; C12N5/06 ; A61K45/00 ; C12Q1/68
摘要：
The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process and a process for preparing them. At least about 70 % of the cells in the cell small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70 % based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the cell small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function, in vitro using the cell colonies enriched with the small hepatocytes which has been induced to be matured.
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