Invention Publication
- Patent Title: KINETIC ASSAY
- Patent Title (中): 动物测定
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Application No.: EP02738989Application Date: 2002-05-14
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Publication No.: EP1404717A4Publication Date: 2006-03-08
- Inventor: COOK CHRISTIAN JOHN , WU YINQIU , MITCHELL JOHN STANTON
- Applicant: HORTICULTURE & FOOD RES INST
- Assignee: HORTICULTURE & FOOD RES INST
- Current Assignee: HORTICULTURE & FOOD RES INST
- Priority: NZ51170501 2001-05-14
- Main IPC: G01N33/53
- IPC: G01N33/53 ; C07J33/00 ; C07J43/00 ; C07K14/76 ; G01N33/531 ; G01N33/543 ; C07K14/77 ; G01N33/72
Abstract:
A hapten-linker-large group conjugate for use in a rapid assay, wherein the assay is kinetic-based not approaching equilibrium, the hapten-linker-large group conjugate being of the general formula: X - W - Y - Zwherein:X is a hapten;W is an optional thioether or ether group;Y is a linker of 10 or more atoms in length; andZ is a large group of sufficient size to provide steric hindrance with respect to the binding of X to a ligand in the absence of Y.Also provided are processes for the production of the conjugates, assay methods and kits. The assays of the invention utilising conjugates of the invention [(5)-OVA] provide a better sensitivity than the same assays with conjugates with shorter linkers [(2)-OVA and 3-OVA].
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