发明公开
EP1404825A2 A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES
审中-公开
A“步行通过”以科技求多核苷酸的体外重组
- 专利标题： A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES
- 专利标题（中）： A“步行通过”以科技求多核苷酸的体外重组
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申请号： EP02747439.4申请日： 2002-06-26
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公开(公告)号： EP1404825A2公开(公告)日： 2004-04-07
- 发明人: SHAO, Zhixin , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert , KENKLIES, Janet
- 申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
- 申请人地址： Sandhofer Strasse 116 68305 Mannheim DE
- 专利权人： Roche Diagnostics GmbH,F. HOFFMANN-LA ROCHE AG
- 当前专利权人： Roche Diagnostics GmbH,F. HOFFMANN-LA ROCHE AG
- 当前专利权人地址： Sandhofer Strasse 116 68305 Mannheim DE
- 优先权： EP01115424 20010627
- 国际公布： WO2003002736 20030109
- 主分类号： C12N9/16
- IPC分类号： C12N9/16
摘要：
A simple and efficient method for random in vitro mutagenesis and recombination of polynucleotide sequences based on walk-through with chain-elongating molecules and chain terminating molecules e.g. dNTPs/ddNTPs followed by reassembly and amplification is described. The utility of this method for improving protein structure and/or function is demonstrated by creating novel sarcosine oxidase variants and by recombining human placental alkaline phosphates and calf intestinal alkaline phosphatase for better mutants with improved properties.
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