发明公开
EP1405910A1 PROCESS FOR PRODUCING HUMAN THROMBIN BY GENE MODIFICATION TECHNIQUE
有权
VERFAHREN ZUR HERSTELLLUNG VON MENSCHLICHEM THROMBIN MITTELS GENMODIFIKATIONSTECHNIK
- 专利标题： PROCESS FOR PRODUCING HUMAN THROMBIN BY GENE MODIFICATION TECHNIQUE
- 专利标题（中）： VERFAHREN ZUR HERSTELLLUNG VON MENSCHLICHEM THROMBIN MITTELS GENMODIFIKATIONSTECHNIK
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申请号： EP02743820.9申请日： 2002-07-04
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公开(公告)号： EP1405910A1公开(公告)日： 2004-04-07
- 发明人: YONEMURA, Hiroshi , IMAMURA, Takayuki , NAKATAKE, Hiroshi , SOEJIMA, Kenji , NOZAKI, Chikateru
- 申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute
- 申请人地址： 6-1, Okubo 1-chome Kumamoto-shi, Kumamoto 860-8568 JP
- 专利权人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute
- 当前专利权人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute
- 当前专利权人地址： 6-1, Okubo 1-chome Kumamoto-shi, Kumamoto 860-8568 JP
- 代理机构： VOSSIUS & PARTNER
- 优先权： JP2001206919 20010706
- 国际公布： WO2003004641 20030116
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12P21/02
摘要：
A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components.
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