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    GENETICALLY MODIFIED ECARIN AND PROCESS FOR PRODUCING THE SAME
    1.
    发明公开
    GENETICALLY MODIFIED ECARIN AND PROCESS FOR PRODUCING THE SAME 有权
    遗传改良剂VERFAHREN ZU DESSEN HERSTELLUNG

    公开(公告)号：EP1405912A1

    公开(公告)日：2004-04-07

    申请号：EP02745822.3

    申请日：2002-07-04

    申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

    发明人： YONEMURA, Hiroshi ,  IMAMURA, Takayuki ,  NAKATAKE, Hiroshi ,  SOEJIMA, Kenji ,  NOZAKI, Chikateru

    IPC分类号： C12N15/12 ,  C07K14/435 ,  C12P21/02

    CPC分类号： C12N9/6418

    摘要： A recombinant ecarin protein that specifically activates prothrombin, said protein being efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transformant microorganism or animal cell transformed with an expression vector in which a gene encoding ecarin is incorporated to the downstream of a promoter so as to produce and accumulate ecarin in culture supernatant or within said transformant and recovering the produced ecarin; and (2) purifying a solution containing the recovered ecarin to obtain purified ecarin. The present invention allows for production of recombinant ecarin on an industrial scale.

    摘要翻译： 用于介导Arg-Ile位点上的凝血酶原-2切割以得到α-凝血酶或用于介导凝血酶原转化的中枢蛋白酶的遗传修饰的ecarin是新的。 还包括用于生产重组ecarin的独立权利要求，其包括：（a）培养通过转化微生物或动物细胞而构建的转化体，所述转化体携带携带启动子下游整合的ecarin的基因的表达载体，并收集所产生和累积的ecarin 在培养基或转化体中; 和（b）回收ecarin进行纯化。 活动：止血。 机制：内肽酶。

    PROCESS FOR PRODUCING HUMAN THROMBIN BY GENE MODIFICATION TECHNIQUE
    3.
    发明公开
    PROCESS FOR PRODUCING HUMAN THROMBIN BY GENE MODIFICATION TECHNIQUE 有权
    VERFAHREN ZUR HERSTELLLUNG VON MENSCHLICHEM THROMBIN MITTELS GENMODIFIKATIONSTECHNIK

    公开(公告)号：EP1405910A1

    公开(公告)日：2004-04-07

    申请号：EP02743820.9

    申请日：2002-07-04

    申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

    发明人： YONEMURA, Hiroshi ,  IMAMURA, Takayuki ,  NAKATAKE, Hiroshi ,  SOEJIMA, Kenji ,  NOZAKI, Chikateru

    IPC分类号： C12N15/09 ,  C12P21/02

    CPC分类号： C12N9/6424 ,  C12Y304/21005

    摘要： A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components.

    摘要翻译： 使用包含培养转化体的基因修饰技术生产人凝血酶，通过用艾卡林处理活化后将回收的人凝血酶原转化为人凝血酶，并从反应混合物中纯化人凝血酶是新的。 使用基因修饰技术生产人凝血酶包括：（a）培养通过将携带编码凝血酶原基因的基因的表达载体转化到动物细胞构建的转化体，所述基因整合到启动子的下游，并将如此产生的凝血酶原聚集在细胞中 收集培养基 （b）通过用艾卡林处理活化后将回收的人凝血酶原转化为人凝血酶; 和（c）从反应混合物中纯化此类人凝血酶。 活动：止血。 没有给出生物数据。 行动机制：无给予。