发明公开
- 专利标题： PRIMERS AND METHOD OF DETECTING BACTERIA
- 专利标题（中）： 底漆和方法细菌检测
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申请号： EP02801540申请日： 2002-10-11
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公开(公告)号： EP1443109A4公开(公告)日： 2005-11-16
- 发明人: NAKANO SHIGERU , KOBAYASHI TORU , FUNABIKI KENICHI , NAGAO YASUHIRO , YAMADA TOSHIHIRO
- 申请人： NISSIN FOOD PRODUCTS LTD
- 专利权人： NISSIN FOOD PRODUCTS LTD
- 当前专利权人： NISSIN FOOD PRODUCTS LTD
- 优先权： JP2001317341 2001-10-15
- 主分类号： C07K14/245
- IPC分类号： C07K14/245 ; C07K14/255 ; C07K14/28 ; C07K14/31 ; C07K14/32 ; C12Q1/68 ; C12N15/09
摘要：
The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.
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