公开(公告)号：EP1443109A4

公开(公告)日：2005-11-16

申请号：EP02801540

申请日：2002-10-11

申请人： NISSIN FOOD PRODUCTS LTD

发明人： NAKANO SHIGERU ,  KOBAYASHI TORU ,  FUNABIKI KENICHI ,  NAGAO YASUHIRO ,  YAMADA TOSHIHIRO

IPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  C12Q1/68 ,  C12N15/09

CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.